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CRL-2095 CAL 27 人舌鱗癌細胞

ATCC^® Number:

CRL-2095™

Price:

Designations:	CAL 27
Depositors:	C Cardona
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	adherent
Organism:	Homo sapiens （human）
Morphology:	epithelial
Source:	Organ: tongue Disease: squamous cell carcinoma
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation:	Isolation date: 1982
Tumorigenic:	Yes
DNA Profile （STR）:	Amelogenin: X CSF1PO: 10,12 D13S317: 10,11 D16S539: 11,12 D5S818: 11,12 D7S820: 10 THO1: 6,9.3 TPOX: 8 vWA: 14,17 CRL-2095 CAL 27 人舌鱗癌細胞
Cytogenetic Analysis:	aneuploid; modal number = 43
Age:	56 years
Gender:	male
Ethnicity:	Caucasian
Comments:	CAL 27 cells were resistant to treatment with VDS （vindesine sulfate）, CDP （cis-platinum） or ACTD （actinomycin D）. A culture submitted to the ATCC in December 1993 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline.
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C
Subculturin
Preservation:	Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature
Doubling Time:	35 hrs
Related Products:	Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2002 recommended serum:ATCC 30-2020
References:	23521: Gioanni J, et al. Two new human tumor cell lines derived from squamous cell carcinomas of the tongue: establishment, characterization and response to cytotoxic treatment. Eur. J. Cancer Clin. Oncol. 24: 1445-1455, 1988. PubMed: 3181269

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CRL-2095 CAL 27 人舌鱗癌細胞