欧美日韩一二区,日韩欧美国产一区二区粉嫩,九九热最新免费在线观看,亚洲精品第一页

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>倉鼠肺細(xì)胞>CRL-1657R 1610 倉鼠肺細(xì)胞

R 1610 倉鼠肺細(xì)胞

簡要描述:CRL-1657 R 1610 倉鼠肺細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻和*培養(yǎng)條件

  • 產(chǎn)品型號:CRL-1657
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2025-04-27
  • 訪  問  量:2494

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-1657 R 1610 倉鼠肺細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻優(yōu)培養(yǎng)條件


CRL-1657 R 1610 倉鼠肺細(xì)胞 的詳細(xì)介紹

CRL-1657

ATCC® Number:



Shipped:  frozen CRL-1657™

Designations:  R 1610

Depositors:   M Rosenberg

Biosafety Level: 1

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Cricetulus griseus (hamster, Chinese)

Morphology: fibroblast



Source: Organ: lung

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Applications: transfection host

Gender:  male

Comments: This line is a clonal derivative of V79 cells (see also V79-4, ATCC CCL-93) that had been treated with ethylmethanesulfonate.

The cells are deficient in galactokinase and are resistant to 2-deoxygalactose and 8-azaguanine.

This is a Chinese hamster somatic cell line.

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing:  Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.



Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Preservation:  Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liqid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

parental cell line:ATCC CCL-93

References: 10111: Schumperli D, et al. Efficient expression of Escherichia coli galactokinase gene in mammalian cells. Proc. Natl. Acad. Sci. USA 79: 257-261, 1982. PubMed: 6281760

25665: Thirion JP, et al. Galactokinase mutants of Chinese hamster somatic cells resistant to 2-deoxygalactose. Genetics 83: 137-147, 1976. PubMed: 1269916

Related Links

NCBI Entrez Search

Make a Deposit

Frequently Asked Questions

Material Transfer Agreement

Technical Support

Related Cell Culture Products






















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
欧美性1生交XXXXX无码| 久久久久久久精品国产怎么下| 一次灌浆与二次灌浆| 福彩3d字谜图谜总汇| 国语自产拍精品香蕉在线播放| 性色AV闺蜜一区二区三区| 经典三级在线观看| 亚洲精品无码高潮喷水a片小说 | 我帮妺妺洗澡忍不住C了她| 美女与野兽图片真人唯美| 国产精品成人久久久久久久| 汽车内饰清洗| 国产SUV精品一区二区88L| 亚洲AV成人无码久久精品老人| 无码视频一区二区三区在线观看| 猛烈顶弄h禁欲老师h春潮| 最近免费中文字幕大全高清10| 大片免费播放器APP| 精品国产乱码久久久久久郑州公司| 欧美酷刑sm在线播放| 老太做爰xxxx| 亚洲夂夂婷婷色拍WW47| 黑人60厘米全进去了| a片免费| 顶级私人家庭影院| 日日天干夜夜狠狠爱| 午夜福利影院| 午夜福利一区二区三区在线观看 | 护士扒下内裤让我爽一夜| 老熟女草bx×| 精品人妻无码专区在线视频| 国产AV无码专区亚洲精品| 乳欲人妻办公室奶水在线电影国产 | 国精产品w灬源码16| xxxx丰满小少妇女自小说| 理论片在线观看免费高清完整版 | 第123章 品尝孕妇滋味| 丰满熟妇乱又伦在线无码视频| 欧美大尺寸SUV欧美| 欧美艳星nikki激情办公室| 老板办公室乳摸gif动态图|