欧美日韩一二区,日韩欧美国产一区二区粉嫩,九九热最新免费在线观看,亚洲精品第一页

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>大鼠正常細(xì)胞>CRL-1442 BRL 3A 大鼠肝細(xì)胞

CRL-1442 BRL 3A 大鼠肝細(xì)胞

簡(jiǎn)要描述:CRL-1442 BRL 3A 大鼠肝細(xì)胞, ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2025-04-27
  • 訪  問(wèn)  量:2173

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-1442 BRL 3A 大鼠肝細(xì)胞, ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)i優(yōu)培養(yǎng)條件


CRL-1442 BRL 3A 大鼠肝細(xì)胞 的詳細(xì)介紹


ATCC® Number:  CRL-1442™

Designations:  BRL 3A

Depositors:   SP Nissley, MM Rechler

Biosafety Level: 1

Shipped:  frozen

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Rattus norvegicus (rat)

Morphology:


Source: Organ: liver

Strain: Buffalo

Cellular Products: somatomedin like multiplication stimulating activity (MSA)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Comments: The serum-free conditioned medium from this cell line is a source of MSA. MSA is a family of polypeptides that can partially satisfy the serum reguirement of chick embryo fibroblasts. [1060]

Propagation:  ATCC complete growth medium: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Growth Conditions: For 24 hrs after initiating culture from a thawed ampule or after subculturing, grow the cells in culture medium with 5% fetal bovine serum. After 24 hrs, the cells may be transferred to serum-free medium.

Subculturing:  Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximay 125 xg for 5 to 10 minutes.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.

Incubate cultures at 37°C.




Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended

Medium Renewal: 2 times per week

Preservation:  Freeze medium: Complete growth medium supplemented with 10% fetal bovine serum and 5% DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

References: 1060: Nissley SP, et al. Proliferation of buffalo rat liver cells in serum-free medium does not depend upon multiplication-stimulating activity (MSA). Cell 11: 441-446, 1977. PubMed: 302146

22582: Coon HG, Weiss MC. A quantitative comparison of formation of spontaneous and virus- produced viable hybrids. Proc. Natl. Acad. Sci. USA 62: 852-859, 1969. PubMed: 4308097

22711: Dulak NC, Temin HM. A partially purified polypeptide fraction from rat liver cell conditioned medium with multiplication-stimulating activity for embryo fibroblasts. J. Cell. Physiol. 81: 153-170, 1973. PubMed: 4735141

22712: Dulak NC, Shing YW. Large scale purification and further characterization of a rat liver cell conditioned medium multiplication stimulating activity. J. Cell. Physiol. 90: 127-130, 1977. PubMed: 833209

22762: Schalch DS, et al. Nonsuppressible insulin-like activity (NSILA). I. Development of a new sensitive competitive protein-binding assay for determination of serum levels. J. Clin. Endocrinol. Metab. 46: 664-671, 1978. PubMed: 755052























產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
成年男人露JIJI网站自慰| 亚洲区小说区图片区qvod| 艳mu1一6全集在线播放| 小说区 图片区色 综合区| 97色情在线观看免费高清| 国产999精品久久久久久| 欧美在线观看| 国产凸凹视频熟女A片| 色五月丁香六月欧美综合| 动漫3d成人h无码国漫| 小鸡庄园每日答案汇总| 国产午夜精华精华精华| 无码国产精品一区二区免费虚拟VR | 99精产国品一二三产区区别| YIN荡纯肉体育生NP男男| 欧美 亚洲 另类 偷偷 自拍| 人妻斩り56歳无码| 国产精品久久人妻无码hd| 婷婷97狠狠成人网站| 亚洲人成色777777精品音频| 国产大片b站免费观看推荐 | 成 人 免费 黄 色 网站视频| 隔壁小寡妇让我爽了一夜| 青苹果乐园在线观看| 色欲久久精品无码一区二区三区| 欧美熟妇另类久久久久久不卡| 久久亚洲精品无码| 10000拍拍18勿入免费看| 未满十八18勿进黄网站| 玩弄懦弱美人双XING大NAI| 国产精品国产三级国产专区53 | 欧美多毛肥胖老妇做爰| √最新版天堂资源网在线| 国产一起色一起爱| 蜜桃狠狠色伊人亚洲综合网站| 亚洲小鲜肉与欧美猛男的区别| 性XXX欧美老妇5060.70| 香港三日本三级少妇三级2021| 国产大陆亚洲精品国产| 母亲とが话しています免费| 啊灬啊灬啊快日出水了A片视频 |