欧美日韩一二区,日韩欧美国产一区二区粉嫩,九九热最新免费在线观看,亚洲精品第一页

咨詢熱線

15000266580

當前位置:首頁 >產(chǎn)品中心>細胞系>人乳腺癌細胞>MDA-MB-468人乳腺癌細胞

人乳腺癌細胞

簡要描述:MDA-MB-468 人乳腺癌細胞
美國傳代
貨號:MDA-MB-468
數(shù)量:大量
生長狀態(tài):正常
運輸方式:常溫
物種來源:人
ATCC Number:HTB-132
細胞類型:普通細胞株-科研實驗
供應商:復祥生物

  • 產(chǎn)品型號:MDA-MB-468
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2025-04-28
  • 訪  問  量:2271

詳細介紹

MDA-MB-468 人乳腺癌細胞

L-15培養(yǎng)基+10%胎牛血清。細胞貨期8-10個工作日

上海復祥生物提供 ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和培養(yǎng)條件,上有細胞照片,歡迎各位老師!

xiangfbio

.


HTB-132 MDA-MB-468

人乳腺癌細胞

ATCC® Number: HTB-132™

Designations: MDA-MB-468

Depositors: R Cailleau

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial


Source: Organ: mammary gland; breast

Disease: adenocarcinoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Isolation: Isolation date: 1977

Applications: transfection host (Roche FuGENE® Transfection Reagents

Nucleofection technology from Lonza)

Receptors: epidermal growth factor (EGF)

transforming growth factor alpha (TGF alpha)

Tumorigenic: Yes

Antigen Expression: Blood Type AB; HLA Aw23, Aw30, B27, Bw35, Cw2, Cw4 (patient)

DNA Profile (STR): Amelogenin: X

CSF1PO: 12

D13S317: 12

D16S539: 9

D5S818: 12

D7S820: 8

THO1: 7

TPOX: 8,9

vWA: 18

Cytogenetic Analysis: modal number = 64; range = 60 to 67.

The cell line is aneuploid human, presumably female (X, abnormal X) with most chromosome counts in the hypotriploid range.; Normal chromosomes X, N2, N3, N7, N8, N10, and N22 are clearly under-represented due to their involvement in the formation of the many marker (19) chromosomes present in this cell line.; A normal chromosome N1 (or two) is identified in each karyotype, but, in addition, regions of chromosome N1 are also present in five different marker chromosomes.; Variation is evident in the normal and marker chromosome copy number from karyotype to karyotype.

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, A

GLO-I, 1-2

Me-2, 1-2

PGM1, 1

PGM3, 2

Age: 51 years

Gender: female

Ethnicity: Black

Comments: The MDA-MB-468 cell line was isolated in 1977 by R. Cailleau, et al., from a pleural effusion of a 51-year-old Black female patient with metastatic adenocarcinoma of the breast. Although the tissue donor was heterozygous for the G6PD alleles, the cell line consistently showed only the G6PD A phenotype.There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution.EGF receptor is present at 1 X 10(6) per cell.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 100%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008

recommended serum:ATCC 30-2020

0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101

Cell culture tested DMSO:ATCC 4-X

References: 1206: Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337

22429: Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779

22544: Pathak S, et al. A human breast adenocarcinoma with chromosome and isoenzyme markers similar to those of the HeLa line. J. Natl. Cancer Inst. 62: 263-271, 1979. PubMed: 283262

22656: Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202

22930: Nigro JM, et al. Mutations in the p53 gene occur in diverse human tumour types. Nature 342: 705-707, 1989. PubMed: 2531845

22977: Bates SE, et al. Expression of the transforming growth factor-alpha/epidermal growth factor receptor pathway in normal human breast epithelial cells. Endocrinology 126: 596-607, 1990. PubMed: 2294006

23111: Avila MA, et al. Quercetin mediates the down-regulation of mutant p53 in the human breast cancer cell line MDA-MB468. Cancer Res. 54: 2424-2428, 1994. PubMed: 8162591

32275: Littlewood-Evans AJ, et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997. PubMed: 9393764

32467: Zamora-Leon SP, et al. Expression of the fructose transporter GLUT5 in human breast cancer. Proc. Natl. Acad. Sci. USA 93: 1847-1852, 1996. PubMed: 8700847






















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯數(shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
欧美群妇大交群| 成品人视频推荐素材网站| 边吃奶边狠狠躁日韩A片| 国产CHINESEHDXXXX| 被吊起来张开腿供人玩弄| 性一交一乱一伦a片| 国产欧美日韩a片免费软件| 被群cao的合不拢腿h纯肉视频| 黑人巨大粗物挺进了少妇| 国产在线拍揄自揄拍无码视频| 国产成人一区二区三区在线观看| 用舌头去添女人下面视频| 无码AV亚洲一区二区毛片| 人妻精品无码中文无码一区无| 人妻出轨和黑人疯狂做国产人| 老太bbwwbbww高潮| 青春禁区在线观看免费国语| 内射人妻无码色AV麻豆| 国产欧美熟妇另类久久久| 免费毛片在线看不用播放器| 西欧FREE性满足HD| 国产AV人人夜夜澡人人爽麻豆| 婷婷大伊香蕉五月天视频| 最近中文字幕大全| 偷偷鲁2020精品偷拍视频| 亚洲精品无码高潮喷水A片软| 久久婷婷激情综合色综合俺也去| 专干老熟女视频在线观看| 久久精品国产久精国产| 蜜桃av噜噜一区二区三区视频| 黎明破晓前电视剧全集免费观看| 777午夜精品久久AV蜜桃| 精品久久久久久无码中文野结衣| 梦精记| 亚洲一卡一卡二新区无人区| 一本久道综合色婷婷五月| 特种兵的又粗又大好爽h| 诱人的大乳bd在线观看| 成人性生交大片免费看试看| 性欧美xxxxx老少交| 国产色欲色欲色欲.www|