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Detroit 562細(xì)胞，人咽頭癌胸水轉(zhuǎn)移細(xì)胞

Organism	Homo sapiens, human
Tissue	pharynx: derived from metastatic site: pleural effusion
Product Format	frozen
Morphology	epithelial
Culture Properties	adherent
Biosafety Level	1
Disease	pharyngeal carcinoma
Age	*****
Gender	female
Ethnicity	Caucasian
Storage Conditions	liquid nitrogen vapor phase

Karyotype Modal number = 64; range = 58 to128
A large subterminal marker chromosome, arm ratio 3:4, is found in 94% of the cells karyotyped. Five to 6 minute chromosomes are present in each cell. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines Clinical Data

Detroit 562細(xì)胞，人咽頭癌胸水轉(zhuǎn)移細(xì)胞

Caucasian

female

Genes Expressed

keratin

Virus Susceptibility Human poliovirus 1
Vesicular stomatitis virus
Comments

The cells are positive for keratin by immunoperoxidase staining.

Complete Growth Medium	The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. *Subction Ratio:** A subction ratio of 1:2 to 1:4 is recommended Medium Renewal:* Every 2 to 3 days
Cryopreservation	Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage Temperature: liquid nitrogen vapor phase
Culture Conditions	Atmosphere: Air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C CCL-138 Detroit 562 人咽頭癌胸水轉(zhuǎn)移細(xì)胞

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Detroit 562細(xì)胞，人咽頭癌胸水轉(zhuǎn)移細(xì)胞