欧美日韩一二区,日韩欧美国产一区二区粉嫩,九九热最新免费在线观看,亚洲精品第一页

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人正常細(xì)胞>CRL-11268 293T/17人胚腎細(xì)胞

CRL-11268 293T/17人胚腎細(xì)胞

簡(jiǎn)要描述:CRL-11268 293T/17人胚腎細(xì)胞
,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2025-04-27
  • 訪  問(wèn)  量:1913

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-11268 293T/17人胚腎細(xì)胞

Permits and Restrictions

View Restrictions

OrganismHomo sapiens, human
Tissuekidney
Product Formatfrozen
Morphologyepithelial
Culture Propertiesadherent
Biosafety Level2 [Cells contain Adeno and SV-40 viral DNA sequences]
Agefetus
Applications

These cells constitutively express the simian virus 40 (SV40) large T antigen, and clone 17 was selected specifically for its high transfectability.  CRL-11268 293T/17人胚腎細(xì)胞

Storage Conditionsliquid nitrogen vapor phase
Derivation

The 293T/17 cell line is a derivative of the 293T (293tsA1609neo) cell line. 293T is a hmperature sensitive gene for SV40 T-antigen was inserted. 293T cells were cloned anighly transfectable derivative of the 293 cell line into which the ted the clones tested with the pBND and pZAP vectors to obtain a line capable of producing high titers of infectious retrovirus, 293T/17.

Antigen Expression

SV40 T antigen

Genes Expressed

SV40 T antigen

Comments

293T/17 cells were cotransfected with the pCRIPenv- and the pCRIPgag-2 vectors to obtain the ANJOU 65 (see ATCC CRL-11269) cell line. ANJOU 65 cells were cotransfected with the pCRIPgag-2 and pGPT2E vectors to obtain the BOSC 23 (see ATCC CRL-11270) ecotropic envelope-expression packaging cell line. ANJOU 65 cells were also cotransfected with the pCRIPAMgag vector along with a plasmid expressing the gpt resistance gene to obtain the Bing (see ATCC CRL-11554)amphotropic envelope-expression packaging cell line.

Complete Growth MediumThe base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.      
SubculturingVolumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.

S

Medium Renewal: Every 2 to 3 days

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
久久A级毛片免费观看| 亚洲色成人一区二区三区小说 | 小雪把衣服解开给老杨看| 亚洲成A人无码亚洲成A无码L| 亚洲AV永久无码精品网址| 国产 亚洲熟妇| 女人下部毛毛高清| 国内少妇偷人精品视频免费| 桥矿影视超棒第1集| 欧美午夜性春猛交XXX| 亚洲精品无码AV人在线观看国产| YIN荡的人妻美妇系列| 免费无码av片在线观看播放| 国产精品刮毛| 国产精品乱码一区二三区| 成 人3D动漫在线观看| 少妇人妻一区二区三区视频| 亚洲精品宾馆在线精品酒店| 午夜精品久久久久久久久 | 出差被绝伦上司侵犯中文字幕| 老师含紧一点H边做边走| 亚洲精品乱码8久久久久久日本| 国产高潮流白浆啊免费a片动态| AV国産精品毛片一区二区| 99久久精品免费看国产| 久久久久夜色精品国产明星| 精品少妇ay一区二区三区| 色一情一乱一伦| 黄色电影免费观看| 进女小姪女体内的视频| 天天干天天射天天操| 女人30如狼40如虎是真的么| 亚洲国产精品久久久久久| 大炕上的性满足| 小峓子的味道4| 人妻少妇精品无码专区漫画| 男男调教后菊撑开扩张A片| 五十度灰完整版| 伦理片在线观看| 极品粉嫩嫩模大尺度无码| 免费AV在线观看|